1/2/2024 0 Comments Who owns wechatLeadership īas Beerens founded the file-sharing platform WeTransfer with Nalden (Ronald Hans) and Rinke Visser in 2009. In January 2022, WeTransfer planned for an IPO but ended up cancelling the offering shortly before listing. In October, WeTransfer announced sales in 2020 of €65m. In April, the WeTransfer Foundation, " Supporting Act", focused on helping emerging creative talent was launched and the company pledged to donate 1% of revenues from 2022 onwards. In March, the 2020 short film " The Long Goodbye" by Aneil Karia and Riz Ahmed commissioned by WeTransfer through WePresent won the 2022 Academy Award for Best Live Action Short Film. In February 2021, WeTransfer achieved carbon-neutral certification having pledged the previous year to reduce emissions by 30% by 2025. In June, the company became a certified B Corporation. In May 2020, India banned the WeTransfer app, citing security reasons. In June 2019, WeTransfer experienced a security incident in which files were "sent to the wrong people." In August, the company closed a €35 million secondary funding round led by HPE Growth. In October, WeTransfer relaunched its mobile app with the name "Collect by WeTransfer". In August, WeTransfer acquired app developer FiftyThree, whose portfolio included sketching app Paper and collaborative presentation app Paste. In January 2018, WeTransfer launched content arm "WePresent". In early 2017, Gordon Willoughby became the company's Chief Executive Officer, taking over from Bas Beerens who became Executive Chairman. In September, WeTransfer opened its first office in the United States at Venice Beach, Los Angeles. In 2016, WeTransfer announced the acquisition of digital design studio Present Plus, established in 2010 by Damian Bradfield and WeTransfer co-founder Nalden. WeTransfer file transfer limit is 200GB for PRO Subscribers as of September 2021 2016–present They also added venture capitalist Troy Carter to its board. In 2015, WeTransfer raised a US$25 million Series A funding round from Highland Capital Partners Europe. These bursaries supported two students from around the globe in their full-time studies. The company initiated its first bursaries that year through a collaborative partnership with Central Saint Martins. In 2014, WeTransfer launched "", an ongoing video series. ![]() In 2013, WeTransfer reached profitability. In 2012, WeTransfer implemented a re-design and introduced a 'Plus' service with support for much larger file transfers (up to 5GB) and storage (50GB) as well as send filed to a maximum of 100 recipients for a price. It was created to enable the sharing of large files (up to 2GB) for free. WeTransfer was founded in 2009 by Rinke Visser, Bas Beerens and Ronald Hans (Nalden) in Amsterdam, Netherlands.
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Further analysis revealed Wnt5a to be differentially regulated during LR and contributed to inhibiting β-catenin activity in primary hepatocytes and liver tumor cells. This suggested that hepatocytes might be a source of Wnt that negatively regulates β-catenin signaling, because Wntless protein is specific for only Wnt secretion. Interestingly, examination of β-catenin signaling, cyclin-D1 expression, and markers of S phase in albumin-cre–driven Wntless knockout (KO) or liver-specific KOs of Wntless ( Wls-LKO) mice revealed temporal prolongation of the hepatocyte proliferative program. ![]() Because β-catenin is a proproliferative signal in LR, identification of what terminates β-catenin signaling might eventually be contributing to cessation of LR. We hypothesized that because a series of proproliferative signaling pathways are turned on to initiate LR soon after PH, identification of negative regulators of such signaling mechanisms may yield clues to the overall LR termination processes. We took an alternative approach to address termination mechanisms of LR. We also provide evidence that the Wnt5a/Frizzled-2 axis suppresses β-catenin signaling in hepatocytes in an autocrine manner, thereby contributing to timely conclusion of the LR process. In conclusion, we demonstrate Wnt5a to be, by default, a negative regulator of β-catenin signaling and hepatocyte proliferation, both in vitro and in vivo. In addition, Wnt5a-LKO hepatocytes failed to respond to exogenous Wnt5a treatment in culture because of a compensatory decrease in Frizzled-2 expression. Intriguingly, Wnt5a-LKO did not display any prolongation of LR because of compensation by other cells. Treatment of primary mouse hepatocytes and liver tumor cells with Wnt5a led to a notable decrease in β-catenin–T-cell factor activity, cyclin-D1 expression, and cell proliferation. Wnt5a expression increased between 24 and 48 hours, and Frizzled-2 between 24 and 72 hours, after PH in normal mice. Wls-LKO showed decreased expression and secretion of inhibitory Wnt5a during LR. This coincided with increased β-catenin–T-cell factor 4 interaction and cyclin-D1 expression. When subjected to PH, Wls-LKO showed prolongation of hepatocyte proliferation for up to 4 days compared with littermate controls. We assessed LR in liver-specific knockouts of Wntless ( Wls-LKO), a protein required for Wnt secretion from a cell. However, how this pathway is turned off when hepatocyte proliferation is no longer required is unknown. 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